Composite

Part:BBa_K4169004:Design

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-09-30)


J23109+B0033+signal peptide PelB with propeptide and mature nattokinase gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 262
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 114
    Illegal AgeI site found at 745
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No.


Source

The part is improved from pelB leader sequence with aprN (without native signal peptide sequence)( BBa_K4060021)

References